You definitely need to draw up a list of your Specification otherwise they'll price cheap for your internal spec and costs will mount during build. Think about:
Type of windows and doors: alu or upvc, double or triple glazed, extra security?
Type of flooring and heating; number of rads.
Inclusive or exclusive of kitchen + appliances?
External and internal doors - standard or something more fancy.
External lighting?
Outside taps and powerpoints?
Landscaping after?
Inclusive or exclusive of skips (clue: INCLUSIVE!)
Type of socket and lighr switch if anything other than bog standard.
Inclusive or exclusive of eg tiling and painting?
Who will do building regs? Will they sort all or will you?
If you've a new bathroom, inclusive or exclusive of sanitaryware? Any complex sanitary installations such as washlet loo, in-wall shower controls/ wall hung taps or basins?
And finally: inc or ex VAT??? Crucial, if ex you can add £££ to the quote.
That's just a few ideas to help you get a good thorough quote.
Have your structural engineers' calcs/ drawings to hand too.