I've attached a picture of DHs comfy pants he wears at night after showering so it doesn't really matter that they are ruined.
But I've no idea what I've done to them! It's not a stain nothing comes off when I scratch. I can't remember what I washed them with but most likely his work clothes (snickers/ hoody) he's a farmer so God only knows what was on his work clothes. I use tesco own brand non bio powder for everyone's clothes.
I don't want to ruin any of my good clothes.
(I do most of the laundry it's what works for us)