Yes.
Way back before I became aware of trans ideology (as opposed to individual trans people), I noticed a sign on an in-store loo. It was next to the 'WOMEN' sign on the outer door, & it read 'Only women & girls to use these toilets'. I mentioned it to DH when I re-joined him, laughing & saying, "Who else would be using it?" & I noticed a female staff member overhear me & how her expression changed. Ah, those innocent days...
The next time I was in there, the hand-written sign had changed to 'FEMALES' with no extra sign on the men's either time, & it was then obvious that a male had been trying to use the women's toilets claiming to be a woman, & the store management were trying to keep him out.
After that the women's toilets were repeatedly out of order while neither the men's nor the disabled ones were affected: someone taking revenge at being excluded, perhaps?
So it happened, even though I was never in the ladies' at the same time as this male.
And really, you have to take into account the 'chilling effect' on women of knowing that their single sex spaces may no longer be single sex. Knowing that you MIGHT encounter a male in, say, a toilet or a rape crisis centre is enough to put women off using them.