Americans overall are against trans women in sports. Here are two recent polls from Gallup and Pew, both prestigious polling orgs in the US.
Gallup, May 2025
A larger majority of Americans now (69%) than in 2021 (62%) say transgender athletes should only be allowed to compete on sports teams that conform with their birth gender. Likewise, fewer endorse transgender athletes being able to play on teams that match their current gender identity, 26%, down from 34%.
Republicans, Democrats and independents are all modestly less supportive of transgender athletes playing on current gender identity teams today than two years ago. The result of these changes is that Democrats are now divided on allowing transgender athletes to play on either male or female teams, while in 2021 more were in favor than opposed. Large majorities of independents (67%) and Republicans (93%) remain opposed to giving transgender athletes a choice of competing on male or female teams.
https://news.gallup.com/poll/507023/say-birth-gender-dictate-sports-participation.aspx
Pew, Feb 2025
A new Pew Research Center survey finds that majorities of U.S. adults favor or strongly favor laws and policies that:
Require trans athletes to compete on teams that match their sex assigned at birth (66%)
Ban health care professionals from providing care related to gender transitions for minors (56%)
Compared with 2022, Americans have become more supportive of laws that limit protections for trans people – and less supportive of laws aimed at safeguarding them.
https://www.pewresearch.org/short-reads/2025/02/26/americans-have-grown-more-supportive-of-restrictions-for-trans-people-in-recent-years/
Here are two other polls from this year that back this up
NBC poll, April 2025
75% of American adults say trans women shouldn't be allowed to play on female sports teams,
https://www.nbcnews.com/politics/politics-news/poll-americans-oppose-trans-women-competing-female-sports-2-3-gen-z-rcna203658
NYT poll, early January 2025
Surveying nearly an equal number of Republicans and Democrats, a poll taken by the New York Times and polling company Ipsos showed that the majority of Americans do not want transgender-identifying men in women’s sports.
Of those surveyed, 79% answered that men “should not” compete in women’s sports when posed with the following question: “thinking about transgender female athletes – meaning athletes who were male at birth but who currently identify as female – do you think they should or should not be allowed to compete in women's sports?”
This number has increased from a 2023 The Center Square Voters' Voice Poll that reported 67% of American voters were collectively against men playing in women’s sports.. . .
When the 2025 New York Times-Ipsos poll is broken up along political divides, 94% of Republicans, 67% of Democrats, and 64% of Independents or “something else” answered that men should not be in women’s sports.. . .
The results of this poll came just before President Trump declared there are only two sexes in America, male and female. The survey was taken from Jan. 2 to 11.
https://www.thecentersquare.com/national/articleb6537968-dff2-11ef-b274-9fbf7250bf7f.html
edit - fix format