As they are both over age and single it is nothing to do with you. Even if they were not single it is nothing to do with you. You need to accept this and move on.
Most people on here have polar opposite opinions. Some are a friend/family member is off limits no matter what. Other are you and they are over, they are not your property and it is none of your business. Each opinion is as valid as the other.
Aside from that. Personally, yuck!!!!!! My younger (by 7 years) sister's ex offered himself to me when their child (my niece) was about 6. Apart from him being too gross to think about it would just have been wrong. The thought of comparisons and doing things they had done, just ew ew ew.
If you sister has always been like this, please take advise from someone with a foul sister don't waste the next 10/15/20 how ever many years hoping one day she will see the light and become a civil person towards you. She won't. Your mother has by her actions let her know that treating you like this is right and acceptable.
Also don't expect your mother to suddenly say you are right your sister is wrong. Again it will never happen. This is how much your family think of you and the role they have assigned you within your family. She could be more sympathetic and understanding of your feelings. It speaks a lot that she isn't
.
I think you need to re-evaluate your relationship with your ex. Is he really a best friend if he can deceive you about this or just omit something this important to you for so long? I would assume he knows you well and how you would react whether you have the right or not. He also does not see things the same way as you in that family and friends are off limits.
Why would he treat you any different from the rest of your family if he as seen you be treated by your sister and this approved of by your mother over the years? He is following their example that you are unimportant in my opinion.
I would cool relations with all of them and make your own way in the world. You are young and have everything ahead of you. People can only treat you this way if you let them.