OP I think you should feel really good about the fact that you’ve communicated your feelings directly to your mum and sister, that shows you’re good at relationships and you’ll be in a good place if you do get in a romantic one. It’s disappointing they haven’t been more responsive but that’s their responsibility, not yours. Without disengaging from individual family relationships, I wonder if it is time to disengage a bit from the overall family dynamic for a month or two and focus on what you can do in the next few years to make your life as good as it can be, partner or no partner, kids or no kids.
If kids are something you really want, is freezing your eggs an option? Solo parenting as some have suggested on here? Adoption? Is it worth researching those options, the pros and cons, and making a plan about what you feel would work for you (including none of those options if they’re not right, but it might be worth researching?) And/or really focus on planning the kind of aunt you’re going to want to be to your nephews and nieces?
Re finding a partner, are you going out as much as you can, saying yes to as many social things as you can, and hitting the dating apps as hard as you can?
Separate from both those things, are there other things you can do to make your life as good as it can be? Promotion at work? Sort out pension/finances? Declutter? Making your home environment lovely? Pet? Travel plans? Fitness goals? New sports? Stuff that makes you feel excited for the months and years ahead? If you don’t have them already, I do think looking out for some female friends in similar situations might help, and then it’s not that you need to disengage from your family, but you will have other dynamics you can key into that feel more engaged with what’s going on with you.
You may well be doing all of this already, but given that you’ve been proactive on the family front, I think the ball is in their court a bit, and maybe it’s time to be proactive about stuff that’s more focused on you.