We've got a 300l tank which might be relevant.
We've just got a nest and have been experimenting with duration of hot water. We had it on 4 hour a day (2 x2 morning and evening) and I reduced it to 2 x 1 with no impact. I just had a shower then bath using the morning's hot as the evening hour is on at 6pm (dying my hair) and it is as hot as ever.
I'm considering reducing to 30 mins each time.
5 of us here, and we don't want a cold wash!