I've wasted a day trying to figure out where I'm going wrong, please can someone save me..
For 0.05 mM I've calculated the change in Abs/min using the equation y=0.0111 x 60 + 0.0234 as shown on the graph. This gives me 0.06894.
Divided this by the extinction coeff. for change in M/min: 3700 = 1.863x10-4 mol/L/min so converted to micromoles by x106 giving 186.32 uM/min
So when I come to calculate the reciprocal for the plot I get 0.00537.
This seems tiny and from other people's graphs it looks like I have an extra 0.
Please someone tell me what I'm obviously missing 