After number 2 definitely (mine are 5 and 9 the same as you) and I'm 42.
I have always had recurrent miscarriages (2 live births from 6 pregnancies) so pregnancy is horrible, anxious misery for me. Despite that I was desperate for number 2.
From when DD was 2 we were trying for another baby and I felt a desperate longing that pushed me on through 2 miscarriages and a scary pregnancy with bleeding and pain throughout. After DS was born I just never felt that way again.
I love our family as it is and it doesn't feel as if there's a vacancy IYSWIM. It's a unanimous view in this house DH and both my DC actively don't want another baby either.
My life has moved on a lot and I just can't see how a newborn would fit in. I have been promoted at work, have more time for hobbies, parents getting older and need me more, love the freedom for a weekend away with DH, kids have their own hobbies that would be harder with a baby.
None of my friends are having any more so we are all at the same stage of life where DC are getting more independent. I just can't see myself at a baby group anymore. I think I'd have nothing in common with first time mums in their 20s.
I still love a cuddle with a newborn but I have no desire at all to change nappies, do night feeds, push a buggy or any of that stuff.
If you do then perhaps it does mean something.