TRVs limit the temperature in the room where they are fitted.
It is correct to have TRVs in all the rooms except the one (usually where you spend most of your time,) where you should have the room stat. The room stat should be in room that does not have other sources of heat, such as a gas fire, a big cooker, or a large sunny window, since this will make it think that the whole house is warm and the boiler can be turned off.
By balancing radiators (that's a different question) you can reduce the rate of heat output in rooms, so that the one in which you have the room stat heats up slowest, and all the others heat up at about the same rate as each other, even though they may have different sized rads. The TRVs will prevent any room getting too warm. You should keep internal doors closed when the heating is on, otherwise all the heat will disappear upstairs and the boiler will keep trying to replace it. The TRV will also cut off unnecessary radiator heat to a room that is hot enough due to, for example, a cooker, a gas fire, or a sunny window, without affecting radiators in other rooms.
It is (sorry) as foolish to turn the boiler on and off yourself, as it would be to not use the thermostat on your oven, and intsead to keep switching in on and off.
It is incorrect to have a TRV in the room where you have the room stat, because it is POSSIBLE for the TRV to turn down the rad in that room, causing the room stat to think the whole house is still cold, and keep running the boiler. If you do have a TRV in the room with the room stat, you should as a minimum turn the TRV to max, or preferably unscrew the thernostatic head from the valve and replace it withe the dummy plastic head ("decorator's cap") that was supplied free with it, or that you can obtain from the manufacturer as you have lost it.
If you have not already got a room stat, you can consider having a "programmable" one which will enable you to set, say, an overnight temperature of 15C between 23:00 and 07:00, a morning temperature of say 20C between 07:00 and 08:30, an unoccupied temperature of 12C between 08:30 and 17:00, an evening temperature of 22C between 17:00 and 23:00 (or any other temperatures and times that take your fancy) with different temperatures and times for Saturdays and Sundays, or other days when you have different activities. You can also (usually) tell it you are going on holiday for nn days, and it will keep the house an an "anti-damp and anti-frost" temperature of your choosing, such as 10C, until the day you are due to come back, when it will go back to your normal daily routine; and if you are staying up late (e.g. for as party), or staying at home all day, e.g. when ill, you can extend the timing, say, for that evening or morning, by 1, 2, or 3 hours, and it will reset to normal schedule the next day. Also, if you have not already got a room stat, you can get a wireless one and carry it around from room to room to see what suits you best (IMO however the chance of wireless faults does add another layer of possible problem)