Hi Ladies, I know that the recommendation nowadays is to make the bottle up 'as and when'. My 6 week old daughter has been suffering badly with colic and therefore we purchased some Dr. Brown anti-colic bottles and started using Colief. To make the bottles in advance the instructions on the Colief packet says that you can make the bottles up, add the Colief (2 drops) and place it in the fridge. The milk can be used within 4 hours and must be used within 12 hours as long as it is kept refridgerated. So why can't milk without Colief be made up in this way? I know several people that make up the bottles for the day and leave them in the fridge. Does anyone else do this? if so have you had any problems with your child? I know that up until several years ago child care professionals said that making up the bottles in advance was fine. So what's changed? It does make life so much easier. Would appreciate your advice as once she stops Colief making the bottles in advance would be far more practical.