Thanks all. Last year i moved from dailies to fortnightly J&J Acuvue Transitions. I have done a LOT of reading with regard to contact lenses , multipurpose fluids, cleaning and in particular AK . From my research i have learned that most multipurpose fluids eg Bausch & Lomb Biotrue and similar ,are ineffective at neutralising acanthamoeba dormant cysts but all can neutralise acanthamoeba trophozoites with an overnight soak.
One methodology known to neutralise acanthamoeba cysts is high temperature exposure applied via microwave.
My methodology is to have three cases which i use in rotation.
Every time my lenses come out i wash and dry hands thoroughly first . I fill a clean case with fresh fluid, extract the lens, place it in palm with some fluid and gently rub then place in case. Repeat with other eye.
Next day before insertion, wash hands and dry thoroughly. Wet fingers with solution. Pick up lens, rinse with solution and insert.
When ive used up all my three lens cases i clean them. I live in a soft water area. I put the three cases and caps in a shallow microwaveable dish. I boil some fresh mains water in a kettle and poor it into the dish so that it covers the cases. I then microwave the bowl and contents in cycles of 2 minutes allowing two minutes in between. I repeat for three cycles so 6 mins worth of microwaving. This generates high temperature water/steam.
I then drain and place the cases and caps onto fresh kitchen roll and leave to air dry for a few hours before use.
If you live in a hard water area you could use filtered or bottled water.
pubmed.ncbi.nlm.nih.gov/11413399/#:~:text=Abstract,Drug%20Administration%20test%20challenge%20microorganisms.