"Was sibling marriage allowed in those times? I can't imagine that it was tbh, so this really baffles me."
Quite simply, no. Actually I don't think it ever has been. In 1540 under Henry VIII there was a law that basically said that marriages were valid unless prohibited in the bible and before that churches largely went by what the bible said anyway.
Back then that even applied to in-laws as well. So, for example, a widower could not marry his dead wife's sister and a widow could not marry her dead husband's brother. The law on this only changed in the 20th century.
There was a whole big thing around Henry VIII marrying Catherine of Aragon because she was his brother's widow, and a widow could not marry her dead husband's brother.
They got round this by Catherine claiming that the marriage had never been consummated and Pope Julius II either was, or affected to be, credulous and gave a special dispensation allowing them to marry.
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But I'd also like to mention a wider point if that's ok?
"...in the early 1500s. I looked at all available records and cross referenced against other trees they appear in."
The first thing I will say about other people's trees on Ancestry (or anywhere else) is to always double check them. Also, you will find that trees are often just copied from other trees and even if it looks like everybody agrees this may well just be a case of everybody just copying one person.
Then you need to consider what the records actually are. I wonder what records have led people to assume a sibling marriage? There aren't too many records from back then.
Let's say that a marriage says that Thomas X marries Elizabeth X - they both have the same surname.
You also see in the records that there was a Thomas X born there 20 years ago to John X and 19 years ago there was an Elizabeth born there to John X.
So perhaps you assume that this is the same Thomas and Elizabeth who married 20 years later?
That isn't a safe assumption to make at all. Extended families often had the same names, a couple will name their child after a brother/sister or aunt/uncle, and you will see the names being repeated in each generation.
For example, in one branch of my family, between the early 1500s and mid 1600s it was very common to see eg Thomas, son of Thomas and grandson of Thomas. They all had brothers called Nicholas and John. And these brothers all had sons called Thomas, Nicholas and John as well.
Likewise with the daughters, Elizabeth (so many Elizabeths!), Edith and Joan.
And they all lived in nearby villages (or even the same village).
Combine this with the fact that not all records survive, so if you suddenly find a Thomas X marrying in the church, that doesn't mean that he was necessarily the same Thomas X that was baptised there (especially if he's supposedly marrying his sister).
Although, of course, it is likely in most cases that the Thomas X baptised in the church is going to be the same Thomas X that gets married in that church 20-25 years later but it's not always the case.
I think it probably more likely that this was a first cousin marriage or second cousin marriage rather than a sibling marriage.
I have two first cousin marriages (eg John X married Elizabeth X) and two second cousin marriages in my tree in the 1600s and also examples of families intermarrying over the course of several generations.
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Just finally, I suggested always double checking other trees. If you have done a DNA test then this can be a very useful check.
For one branch of my tree going back very early I had to rely on one particular tree based on research done earlier and published in the 1990s. I have since managed to corroborate quite a bit of it, but certainly not all.
However, I have found a number of DNA matches that only make sense if the tree is accurate. This does give me a lot more faith that the tree is accurate. The DNA common ancestors are quite a long way back but there was some endogamy (the same families intermarrying) in both branches.
Basically, one branch of the family went off to America and became tobacco farmers in the 1650s and my branch stayed behind as farm workers.
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So, I would be doubtful if this was an actual sibling marriage.