I used to work for a publisher a few centuries ago, and IIRC they prefer to receive manuscripts from agents rather than from authors, but many, especially the smaller ones, will still read manuscripts sent in by authors.
The accepted format is typed on A4, one side only, double or one-and-a-half line spacing. 10 or 12 point in a standard font. My personal preference is for 10pt Arial or 12pt TNR. Not critical but helpful, is to right-justify the text and indicate new paragraphs by indenting the first line. Start chapters on a new page.
Run off several copies if you can (use a print shop, it's the cheapest way) because the ms will get tatty, and will show any reader that you have submitted it unsuccessfully to other publishers.
If it's a large novel, then you might find it cheaper (and the publishers often prefer it) if you send only the first few chapters.
I don't remember what preferences there are over the binding, but if the document is relatively short (eg only a few chapters) then a hole punched through the top left corner and a ring or treasury tag threaded through is fine. If it's thicker, then you'll need something more substantial, like a soft ring binder. Try to keep it as unbulky and as easy-to-handle as possible. The title page and back cover should be of light card or slightly stiffer paper for this reason.
In your covering letter, DO NOT SAY '...enclosed for your perusal.' Every editor I have known hated that phrase!
This is my experience, but it may be out-of-date given new technology. It was only 10-15 years ago, but since then computers are so much more common, that it's possible publishers may now prefer ms on cd.
Good luck!!