Congratulations on both your new baby and on organising a night out!
You can defrost breastmilk in advance in the fridge and have your parents give the bottles over the time you're away. I would keep the defrosted milk for up to 24 hours in the fridge.
I wouldn't reuse a bottle that your baby has fed from, as he will pass bacteria from his mouth back into the bottle during the feed. But if the 250ml is defrosted, you could pour it into different bottles before the feeding begins so that it isn't wasted. The guideline is that a baby over one month will take about 24oz per 24 hours, though this will vary for each child, and I wod recommend offering this in 2oz/3oz bottles to avoid waste.
Yes, it is fine to defrost a bag of milk under the tap and use it straight away. I would only keep milk defrosted this way for a few hours though, so have them defrost any large bags in the fridge (to use gradually) and any small bags under the tap for immediate consumption.
The formula rules about milk storage don't apply to breastmilk. It is fine to store it in the fridge, it certainly doesn't need to be heated to 70 degrees to kill bacteria (in fact heating breastmilk beyond body temp will destroy some of the nutritional value) and the volumes likely to be drunk are smaller.
If I was planning your night out, I would leave 1oz per hour defrosted in the fridge (use your big bags to deforest this amount, and it's fine for mix the bags once defrosted) and instructions to defrost more bags in the fridge if they think they're going to run out (they might spill a bottle, baby might feed a lot, they might warm too much at each feed and waste a lot) or under the tap if they need more milk now. If you can trust your parents to streralise bottles/only use a steralised stash, then I would leave the milk in the bags it was frozen in to defrost in the fridge and let your parents fill the bottles from the bags. This means that they can adjust the volume of each feed as they learn about your babies bottle-feeding preferences, and the milk moves between containers the fewest times.
It would also be sensible to plan a few afternoons out without baby to see whether your parents are able to persuade your baby to take a bottle.
If you feel very strongly that baby shouldn't have formula, then make this very clear to your parents. There are so many stories of grandparents giving formula because they think it's easier than faffing about defrosting milk, and mostly it all seems very well intentioned and the grandparent has no idea formula will upset the mother. Just be crystal clear about what you want them to do.
Kellymom.com has lots of great advice about expressing and stroring breastmilk.
Good luck!