I am a few year older than you. I do think women are discriminated against if they look their age. I once worked with another professional woman and she had thin, grey hair cut short. I thought she was consistently undervalued because as far as I could tell, she was good at her job. As a result, I stay skinny as a size 8, keep my roots coloured, have a light touch of botox and filler, had my bunions fixed and dress youthfully (in a tasteful way).
I am involved in recruiting people on a fairly regular basis and I have had only a few people be downright unpleasant or domineering in an interview. I would never employ those people. I don't want to hire a management challenge no matter how good they are at other aspects of the job. People who give thoughtful answers - not overlong ramblings - and appear flexible and pleasant - are at an advantage. Smiling helps. Also, tailor your answers to the interviewer. Don't give a very technical answer to somebody who doesn't understand the technicalities of the job. Try to look interested and lively yet respectful.
I have worked with people who were seen as ball breakers. Some of them were just very big and loud people. I think it is really important for women to control their voice and keep the volume appropriate. I know this stuff shouldn't matter and men wouldn't be seen in the same way but you want to get employed. We always ask how you would handle a disagreement - I work in a technical field - and the answer should centre around understanding the other person's point of view, coming to a compromise, discussing with some other experienced colleagues etc.
I have had people be condescending to me when they didn't realise my age or seniority. Never be this person as I'll have just got a very clear idea of how you might treat younger people as well as me. Also be a delight to the secretaries or PAs who may interact with you.
They won't ask but try to convey that whatever family reasons meant you weren't working are no longer a problem. Express nothing but the most profound admiration for any former colleagues or employers unless they have actually been found guilty on criminal charges.