I think it depends on the trip.
Things I do:
One bag that for all shoes so I’m not separating them from the clean clothes. I usually use a big cloth beach bag so it can be washed after the trip.
One bag for all toiletries.
When DCs were younger I’d take everything including kitchen bowl, I would use it for washing up, warming bottles, sterilising bottles, keeping things in the room tidy, but it was also great for separating packing in the case. I’ve also taken large Tupperware containers for the same thing. I use smaller Tupperware containers with lids for in room food or food on the go, they can be washed and reused throughout the trip.
Plug in cool boxes are brilliant for keeping food & drink chilled. Saves a fortune on buying food if we have fresh food to make lunches in the hotel. Or just a normal cooler and buy bags of ice.
I regularly take old clothes/underwear/shoes/toiletries that are ready to be thrown away and just get rid of them when finished with rather than bringing them back with me.
One bag is always dedicated to laundry, big summer beach bags are usually my go to bag so they can be washed too.
if we are moving around a lot, for example one night stop offs I will prepare an overnight bag with everything we all need in one bag. It means we don’t need to fully unpack the car or if we do we only need to use what’s in the one bag. We like road trips so have gotten good at splitting our packing so we are not having to open everything up all the time.
I read a tip on using those hanging clothes storage things to keep toiletries and other things organised when travelling. It was ok, good if staying somewhere where you don’t have much space, but I found it more hassle that it was worth. Just as easy to keep all my toiletries in the one bag.