You can tell from the pronouns Joan but if I recall the pronouns are not used that often (at least in the original AA Milne) but rather the names, which are not very gender specific are used. The characters is also don't seem to have traits which are portrayed as being gender specific. Apart from perhaps Christopher Robin.
Eeyore is quite intelligent, miserable, pessimistic and a bit rude to others, but somehow lovable
Piglit is small with a high-pitched voice, loyal, friendly, quite clever, brave and adventurous, occasionally scared of Tigger
Tigger talks about his self a lot, is boastful, is childlike (and has to be cared for by Kanga), is a fussy idea, is confident, outgoing and friendly though sometimes scares others with his enthusiasm. Talks a lot.
Winnie himself is caring, friendly, comfort eats and puts himself down
I think the main characters have a mixture of traits that are not all generally thought of as particularly masculine.