I started reading, but didn’t reach the end. All these threads are designed to make the science sound so complex that credulous people will conclude they don’t understand and that therefore they must take the word of the much cleverer person as truth.
Biological sex existed long before we knew anything about chrosmosome function. Information we gain about chromosomes has largely backed up the observation that there are two sexes. The exact interplay of how chromosomes function and variation withi the two sex groups that sometimes overlap does not affect the simple fact that there are males and females and that for sexual reproduction to occur, one of each of those is required.