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AIBU?

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Original poster

To ask people who are sane if there is a line…

36 replies

ConfusedConnie9 · 13/01/2025 16:55

I am due AF a Friday and these have all been very very clearly BFN up to this last one and now I’m not sure if my eyes have lost their minds!!
i have had excruciating breast pain all weekend which I never have as well as some cramps so took a few clearblue early tests over the weekend but also BFN

Very confused

OP posts:
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ShelfyElfy25 · 14/01/2025 10:49

Lackinginspiration1 · 14/01/2025 10:09

these tests use gold particles coated with antibodies, which can aggregate if the pH changes too much, such as if you have too much urine on the strip or dip too deep so that the urine itself touches the membrane rather than the sample pad. That aggregation makes the gold particles clump together so they don’t move up the membrane as well, and can get caught by the molecules on the test line even if there’s no hCG present- that’s called nonspecific binding. It’s not very common though as these tests are developed to try and prevent that happening, so false positives with red tests are more likely to be a chemical pregnancy I.e. where the egg is fertilised but then doesn’t implant properly. The blue tests use nitrile rather than gold particles- nonspecific binding is much more of a problem with those so false positives are more likely

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That's so interesting, thank you for such an amazing explanation ☺️

Janey3090 · 14/01/2025 10:29

Sorry OP I don't see anything on these tests but it's still early! Test over the weekend if AF doesn't arrive on Friday x

TheKeatingFive · 14/01/2025 10:12

You're still very early to test. I went through this torture, it's not worth it. Test next Monday if your period hasn't arrived.

Lackinginspiration1 · 14/01/2025 10:09

ShelfyElfy25 · 14/01/2025 09:48

Just out of interest, please could you explain why flooding the test leaves a line? ☺️

Edited
Show quote history

these tests use gold particles coated with antibodies, which can aggregate if the pH changes too much, such as if you have too much urine on the strip or dip too deep so that the urine itself touches the membrane rather than the sample pad. That aggregation makes the gold particles clump together so they don’t move up the membrane as well, and can get caught by the molecules on the test line even if there’s no hCG present- that’s called nonspecific binding. It’s not very common though as these tests are developed to try and prevent that happening, so false positives with red tests are more likely to be a chemical pregnancy I.e. where the egg is fertilised but then doesn’t implant properly. The blue tests use nitrile rather than gold particles- nonspecific binding is much more of a problem with those so false positives are more likely

ShelfyElfy25 · 14/01/2025 09:48

Lackinginspiration1 · 14/01/2025 09:02

I make these types of tests. Stick with the red ones, cheap is perfectly good. Digital ones are less sensitive. But you are testing very early when hCG levels will be low and will fluctuate depending on how concentrated your urine is. So just wait a few days, be patient and test ideally with first morning urine, but be prepared that it may have been a chemical pregnancy. Good luck!

Just out of interest, please could you explain why flooding the test leaves a line? ☺️

Lackinginspiration1 · 14/01/2025 09:02

I make these types of tests. Stick with the red ones, cheap is perfectly good. Digital ones are less sensitive. But you are testing very early when hCG levels will be low and will fluctuate depending on how concentrated your urine is. So just wait a few days, be patient and test ideally with first morning urine, but be prepared that it may have been a chemical pregnancy. Good luck!

Original poster
ConfusedConnie9 · 14/01/2025 08:43

Probably invisible as only visible to me in certain lighting!

To ask people who are sane if there is a line…
OP posts:
Original poster
ConfusedConnie9 · 14/01/2025 08:42

The faintest line ever on the next cheapie

Not even enough to be seen in a picture although I’ll attach it anyway - hard to know if that’s an evap line as so faint although haven’t had any evap lines on these cheap tests before

OP posts:
TheWonderhorse · 14/01/2025 07:49

ConfusedConnie9 · 14/01/2025 07:30

This must have been what happened then 😢 thank you

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Not necessarily. Try another cheapie but dip for less time. You only need enough to soak up past the line.

Hang in there, I know it's hard going ❤️

Original poster
ConfusedConnie9 · 14/01/2025 07:47

EvangelicalAboutButteredToast · 14/01/2025 07:32

I always found those cheap tests pretty accurate. If you’ve done a few and when dried there’s a clear line there id say you have every reason to be hopeful.

Sadly this morning the exact same test couldn’t look more negative
So confusing

OP posts:
EvangelicalAboutButteredToast · 14/01/2025 07:32

I always found those cheap tests pretty accurate. If you’ve done a few and when dried there’s a clear line there id say you have every reason to be hopeful.

Original poster
ConfusedConnie9 · 14/01/2025 07:30

ShelfyElfy25 · 14/01/2025 07:23

Yes it can cause a false positive if you dip for too long or put too much urine on the test.

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This must have been what happened then 😢 thank you

OP posts:
ShelfyElfy25 · 14/01/2025 07:23

ConfusedConnie9 · 13/01/2025 18:20

Would dye bleeding over make a line like that?
i dipped in a cup

Yes it can cause a false positive if you dip for too long or put too much urine on the test.

kikisparks · 14/01/2025 07:12

ConfusedConnie9 · 14/01/2025 07:10

Negative digital 😢

Digitals are not very sensitive, can you get a first response to take tomorrow?

Original poster
ConfusedConnie9 · 14/01/2025 07:10

Negative digital 😢

OP posts:
Original poster
ConfusedConnie9 · 13/01/2025 20:04

I’ll get a digital test tomorrow
I did wait long enough - 5 minutes exactly
The line is still there and pink but I’ll get another one tomorrow

OP posts:
Mercedes45 · 13/01/2025 19:29

Get a digital test

Errors · 13/01/2025 19:27

To me it doesn’t look like you waited long enough to read the result as it has dye all over it. You need to do another

PerditaLaChien · 13/01/2025 19:23

You need to get a first response they are honestly just miles better.

partygarden · 13/01/2025 19:21

Yes I can see a line!

Original poster
ConfusedConnie9 · 13/01/2025 18:20

Would dye bleeding over make a line like that?
i dipped in a cup

OP posts:
NotGottaClue · 13/01/2025 17:50

Unsure if it's dye bleeding over
Get a first response.

ShelfyElfy25 · 13/01/2025 17:50

Looks like it's flooded to me so I wouldn't take it as accurate

FrannyScraps · 13/01/2025 17:49

It looks flooded. Pee in a cup and dip the test.

BlondeMamaToBe · 13/01/2025 17:48

It looks like the dye is bleeding all over. I wouldn’t see it as valid.